Spontaneously Ruptured Spleen Samples in Patients With Infectious Mononucleosis

Analysis of Histology and Lymphoid Subpopulations

Marcos M Siliézar, MD; Catuxa Celerio Muñoz, MD; Jon Danel Solano-Iturri, MD; Laura Ortega-Comunian, MD; Manuela Mollejo, MD; Santiago Montes-Moreno, MD; Miguel A Piris, MD


Am J Clin Pathol. 2018;150(4):310-317. 

In This Article

Materials and Methods

Paraffin-embedded blocks from seven formalin-fixed, paraffin-embedded cases were stained for H&E and for a panel of antibodies (listed in Table 1).

Clonality was analyzed by polymerase chain reaction (PCR) to detect immunoglobulin H (IgH) and TCR gene rearrangements by standard methods.[8] It was assayed by conventional methods using DNA extracted from formalin-fixed, paraffin-embedded tissue. Multiplex PCR to detect clonal VH-JH rearrangements was performed using standardized Biomed2 primers, as fully described elsewhere.[9] We analyzed complete rearrangements of IgH (VH FR1-JH, VH FR2-JH, VH FR3-JH) and rearrangements of TCR (TCRG tube A [Vg-Jg], TCRG tube B [Vg-Jg], TCRB tube A [Vb-Jb], TCRB tube B [Vb-Jb], TCRB tube C [Db-Jb]). Results were interpreted following the recommendations of the Euroclonality/Biomed2 group.[10]