Future proteomics studies on ZGs with advanced methodologies and increased sensitivity will certainly further increase the number of potential ZG proteins identified (e.g., small proteins, regulatory peptides and high-molecular-mass proteins). The confirmation of the identified proteins will represent a major task in the near future. However, only a thorough functional analysis of the identified 'players' involved in ZG biogenesis/secretion will help to elucidate the mechanisms leading to pancreatic and/or intestinal diseases and to develop new therapeutic strategies. Valuable input may come from quantitative proteomics studies of patients and control individuals, the creation of a protein interaction network, and the elucidation of the role of post-translational modifications (e.g., glycosylation and phosphorylation).
The authors would like to thank Horst F Kern (University of Marburg, Germany) for stimulating discussions, and they apologize to those whose work has not been cited owing to space limitations. The authors thank Brigitte Agricola and Volkwin Kramer (Marburg, Germany) for excellent technical assistance.
Financial & competing interests disclosure
This work was supported by the German Research Foundation (DFG, SCHR 518/5-1, 2), the J Manchot Foundation (Düsseldorf, Germany), the Portuguese Foundation for Science and Technology (FCT [SFRH/BD/38629/2007 (to Cornelia Rinn), SFRH/BPD/37725/2007 (to Maria Gomez-Lazaro), SFRH/BD/48722/2008 (to Miguel Aroso)]), and the University of Aveiro. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.
No writing assistance was utilized in the production of this manuscript.
Expert Rev Proteomics. 2010;7(5):735-747. © 2010
Expert Reviews Ltd.
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