Proteomic Analysis of Zymogen Granules

María Gómez-Lázaro; Cornelia Rinn; Miguel Aroso; Francisco Amado; Michael Schrader


Expert Rev Proteomics. 2010;7(5):735-747. 

In This Article

Five-year View

Future proteomics studies on ZGs with advanced methodologies and increased sensitivity will certainly further increase the number of potential ZG proteins identified (e.g., small proteins, regulatory peptides and high-molecular-mass proteins). The confirmation of the identified proteins will represent a major task in the near future. However, only a thorough functional analysis of the identified 'players' involved in ZG biogenesis/secretion will help to elucidate the mechanisms leading to pancreatic and/or intestinal diseases and to develop new therapeutic strategies. Valuable input may come from quantitative proteomics studies of patients and control individuals, the creation of a protein interaction network, and the elucidation of the role of post-translational modifications (e.g., glycosylation and phosphorylation).