What is the role of immunofluorescent microscopy in the workup of herpes simplex virus (HSV) infection?

Updated: Feb 27, 2019
  • Author: J Michael Klatte, MD; Chief Editor: Russell W Steele, MD  more...
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Answer

A slide prepared from scrapings of lesions may be examined for herpes simplex virus antigens by using immunofluorescent microscopy. Herpes simplex virus type-specific monoclonal antibodies, which are available in commercial antibody staining kits, permit the identification and typing of isolates in tissue samples. Slides containing cells from suspected herpes simplex virus lesions or specimens should be fixed with ethanol or methanol and immediately transported to the laboratory for analysis.


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