How is the two-glass test performed for the diagnosis of chronic bacterial prostatitis (CBP)?

Updated: May 25, 2021
  • Author: Samantha D Kraemer, MD; Chief Editor: Edward David Kim, MD, FACS  more...
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The two-glass test, also known as pre-massage and post-massage test, is simple and cost-effective. It is used more commonly in clinical practice to screen patient with prostatitis. The pre-massage urine specimen is midstream catch and the post-massage urine specimen is the initial 10 mL after prostatic massage. The sediment is evaluated with microscopy and the urine is cultured. See the image below.

Urine culture with greater than 100,000 colony-for Urine culture with greater than 100,000 colony-forming units (CFU) of Escherichia coli, the most common pathogen in acute and chronic prostatitis. Chronic bacterial prostatitis must be confirmed and diagnosed using a urine culture.

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